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Title   발암제 N-nitrosomorpholine 에 의하여 Preneoplastic Reprogram 된 쥐간세포의 핵대사에 관한 연구 ( N-nitrosomorpholine Directed Preneoplastic Reprogramming of Nuclear Metabolism of Rat Hepatocytes )
Publicationinfo   2000 Jan; 032(06): 1075-1084.
Key_word   N-nitrosomorpholine , Preneoplastic reprogram , Nucleolar signals , Heat shock resistance
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Abstract   Purpose: An attempt was made to investigate N-nitrosomorpholine (NNM) induced carcinogenic processes in rat liver. Materials and Methods: Rats were fed with NNM (200 mg/1) for 7 weeks, after then stopped. Length of telmere and activity of telornerase were analyzed. Hepatocytes were isolated and grown on tissue culture. Heat shock was treated at 43"C, and patterns of cell death ere evaluted by fluorescent study. Nuclei and nucleoli were isolated for analysis of various signal molecules. Results: Shortening of telomere length presented in NNM treated liver, but induction of telomerase was not found. Ex vivo hepatocytes from 10-12th week showed increased heat shock resistance at 43"C. NNM-treated hepatocytes exhibited heat shock induced cell death (necrosis) after 7 hours, whereas the control showed necrosis after 3 hours. The signal molecules related to nucleolar growth revealed increased expression which included B23, C23, p38, Erkl/2 and p120. Partial degradation of B23 and Erk2 was noted in necrosis of NNM treated hepatocytes induced by heat shock. Conclusion: The hepatocytes at the stage of 10-12th week in the stop experiment of NNM are situated in the tumour promotion. Those cells showed various metabolic alterations. We found that the increased growth related signals were accompanied with increased heat shock resistance, telomere shortening but no induction of telomerase.
저 자   정진숙(Jin Sook Jeong),김민찬(Min Chan Kim),최용천(Yong Chun Choi),김상순(Sang Soon Kim),정갑중(Ghap Joong Jung)